fluorescence confocal microscopy Search Results


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Medizinische Hochschule Hannover confocal fluorescence microscopy
Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Carl Zeiss confocal laser scanning microscopy zeiss lsm pascalexciter fluorescence system
Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser <t>fluorescence</t> <t>microscopy.</t> BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .
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Image Search Results


Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser fluorescence microscopy. BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .

Journal: European Journal of Biochemistry

Article Title: Analysis of the thyrotropin‐releasing hormone‐degrading ectoenzyme by site‐directed mutagenesis of cysteine residues

doi: 10.1046/j.1432-1327.2000.01277.x

Figure Lengend Snippet: Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser fluorescence microscopy. BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .

Article Snippet: We thank André Wolfstein and Beate Sodeik (Institut für physiologische Chemie, Medizinische Hochschule, Hannover) for experienced support with confocal fluorescence microscopy, Lutz Schomburg for advice and helpful discussions, Uwe Grunenberg for excellent technical assistance, Valerie Ashe for linguistic help and the Deutsche Forschungsgemeinschaft for financial support.

Techniques: Fluorescence, Microscopy, Transfection, Plasmid Preparation, Mutagenesis